Evaluation and analysis of SUWAN-1-SR Escherichia coli-mediated acetone-butanolethanol fermentation using roasted corn cob species for biobutanol synthesis

J. E. OSSAI; F. O. OSHOMOGHO

J. E. OSSAI University of Benin
F. O. OSHOMOGHO University of Benin

Keywords: Biobutanol, ABE fermentation, Escherichia coli, SUWAN-1-SR roasted corn cob, Response Surface Methodology

Abstract

The Objective of this paper was to describe and assess the SUWAN-1-SR species
of roasted corn cobs for the acetone-butanol-ethanol fermentation process using
Escherichia coli to produce biobutanol. The feedstock percentage content of
cellulose, hemicellulose, and lignin was estimated, with values of 43.65%, 51.50%,
and 4.85%, respectively. Variations in elemental composition were found when the
SUWAN-1-SR Species of maize cobs were characterized; potassium had the
highest weight percentage of 0.75 and a of peak 4143 cp/Ma The Response surface
model optimization showed how time 27.1978 minutes, temperature of 80.69810C
and a maximum concentration of 0.4623mol/dm3 were adjusted based on the
model developed from each response and gave the percentage total sugar yield of
1489.88 g/l for SUWAN-1 SR species of roasted corn cobs, the adjusted R2 value
was 0.9054. HPLC analysis was also performed to determine the concentration of
biobutanol produced from the ABE fermentation process.